5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide jẹ agbo-ara ti a lo ni ọpọlọpọ awọn ẹkọ-ẹrọ biokemika, ni pataki fun wiwa ati iworan ti iṣẹ ṣiṣe enzymu.O jẹ sobusitireti ti o le jẹ hydrolyzed nipasẹ awọn ensaemusi kan pato, ti o yọrisi itusilẹ ti ọja awọ tabi Fuluorisenti.
Apọpọ yii ni a lo nigbagbogbo ni awọn idanwo lati rii wiwa ati iṣẹ ṣiṣe ti awọn enzymu bii beta-galactosidase ati beta-glucuronidase.Awọn enzymu wọnyi ya awọn ẹgbẹ acetyl ati glucosaminide kuro ninu sobusitireti, eyiti o yori si dida chromophore buluu tabi alawọ ewe.
Ẹya alailẹgbẹ ti 5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide ngbanilaaye fun wiwa irọrun ati iwọn iṣẹ ṣiṣe henensiamu.Lilo rẹ ni ọpọlọpọ awọn ilana idanwo, pẹlu histochemistry, immunohistochemistry, ati awọn igbelewọn ti o da lori sẹẹli, ti ṣe alabapin si oye ti o dara julọ ti awọn iṣẹ enzymu.