Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside CAS:24404-53-3

Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside jẹ lilo bi sobusitireti fun henensiamu β-galactosidase.Nigbati β-galactosidase ba ṣiṣẹ lori rẹ, agbo naa jẹ hydrolyzed, ti o mujade iṣelọpọ p-nitrophenol tabi o-nitrophenol, eyiti o ni awọ ofeefee ti o le rii ni irọrun.Eyi jẹ ki o wulo bi sobusitireti fun awọn igbelewọn ti o wọn iṣẹ ṣiṣe β-galactosidase.

Ohun elo kan ti o wọpọ ti Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside wa ninu awọn idanwo jiini onirohin.Ninu awọn igbelewọn wọnyi, apilẹṣẹ ti iwulo pọ mọ jiini onirohin, gẹgẹbi jiini beta-galactosidase.Iṣẹ ṣiṣe ti jiini onirohin lẹhinna ni abojuto nipasẹ wiwọn iṣẹ ṣiṣe β-galactosidase nipa lilo Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside bi sobusitireti.Eyi ngbanilaaye awọn oniwadi lati ṣe iwadi ikosile pupọ ati ilana.

Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside tun le ṣee lo lati ṣe ayẹwo fun awọn inhibitors tabi awọn oluṣe ti β-galactosidase.Nipa idanwo awọn agbo ogun oriṣiriṣi tabi awọn oludije oogun, awọn oniwadi le ṣe idanimọ awọn ohun elo ti o ṣe atunṣe iṣẹ ṣiṣe ti enzymu yii, eyiti o le ni awọn ohun elo itọju ailera ni awọn arun tabi awọn ipo kan.

Ni afikun, agbo-ara yii le ṣee lo ni isọdọmọ amuaradagba.β-galactosidase nigbagbogbo ni idapọ si awọn ọlọjẹ ti iwulo lati dẹrọ wiwa ati isọdi wọn.Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside le ṣee lo bi sobusitireti si iboju ati sọ di mimọ awọn ọlọjẹ idapọ wọnyi nipa wiwa iṣẹ ṣiṣe β-galactosidase.