4-NITROPHENYL-ALPHA-D-MANNOPYRANOSIDE CAS:10357-27-4

Ni pato, o jẹ lilo nigbagbogbo fun wiwa ti iṣẹ-ṣiṣe beta-mannosidase.Nigbati 4-nitrophenyl-alpha-D-mannopyranoside ti wa ni cleaved nipasẹ beta-mannosidase, o tu ọja awọ-ofeefee ti a npe ni 4-nitrophenol.Awọn kikankikan ti awọn awọ ofeefee ni taara iwon si iye ti beta-mannosidase aṣayan iṣẹ-ṣiṣe ti o wa ninu awọn sample.Ayẹwo lilo 4-nitrophenyl-alpha-D-mannopyranoside ti wa ni igba ti a lo lati iwadi enzymu kinetics, wiwọn enzymu aṣayan iṣẹ-ṣiṣe awọn ipele, ati iboju fun. mutanti tabi awọn oludena ti beta-mannosidase.Apapọ yii n pese ọna iyara ati irọrun lati ṣe iwọn iwọn iṣẹ ṣiṣe ti enzymu kan pato.