4-Nitrophenyl-alpha-D-galactopyranoside CAS:7493-95-0

Sobusitireti fun awọn igbelewọn enzymatic: 4-Nitrophenyl-alpha-D-glucopyranoside jẹ lilo nigbagbogbo bi sobusitireti ni awọn idanwo enzymatic lati wiwọn iṣẹ ṣiṣe ti glycosidases ati awọn enzymu miiran ti o ni ipa ninu iṣelọpọ carbohydrate.Awọn enzymu wọnyi le sọ adehun laarin glukosi ati ẹgbẹ 4-nitrophenyl, idasilẹ ọja ofeefee kan ti a pe ni 4-nitrophenol.Awọn iye ti 4-nitrophenol ti ipilẹṣẹ ni taara iwon si awọn enzymu aṣayan iṣẹ-ṣiṣe, gbigba fun awọn pipo wiwọn ti henensiamu aṣayan iṣẹ-ṣiṣe.

Ṣiṣawari iṣẹ ṣiṣe enzymu: Nipa lilo 4-Nitrophenyl-alpha-D-glucopyranoside bi sobusitireti, iṣẹ ṣiṣe ti awọn enzymu kan pato le ṣe iwọn.Fun apẹẹrẹ, awọn enzymu glycosidase gẹgẹbi beta-glucosidase, alpha-glucosidase, tabi beta-galactosidase le fa agbopọ mọ, ti o tu 4-nitrophenol silẹ, eyiti o le rii ni photometrically.Ọna yii jẹ lilo pupọ ni ọpọlọpọ iṣoogun, oogun, ati awọn ohun elo imọ-ẹrọ.

Ṣiṣayẹwo giga-giga: Nitori ibamu rẹ pẹlu awọn ọna ṣiṣe wiwa adaṣe, 4-Nitrophenyl-alpha-D-glucopyranoside ni a lo nigbagbogbo ni awọn igbelewọn ibojuwo-giga.Apapọ yii ngbanilaaye fun isọdi iyara ati lilo daradara ti iṣẹ ṣiṣe enzymu, ṣiṣe ni iwulo ninu iṣawari oogun, imọ-ẹrọ enzymu, ati awọn ikẹkọ idinamọ enzymu.

Awọn ohun elo iwadii: 4-Nitrophenyl-alpha-D-glucopyranoside le ṣee lo ni awọn ohun elo iwadii lati rii wiwa tabi iṣẹ ṣiṣe ti awọn enzymu kan pato ti o ni ibatan si awọn arun pupọ.Fun apẹẹrẹ, iṣẹ ṣiṣe ti awọn enzymu kan le ṣee lo bi ami idanimọ fun awọn arun kan pato, ati 4-Nitrophenyl-alpha-D-glucopyranoside le ṣee lo bi sobusitireti lati wiwọn iṣẹ ṣiṣe ti awọn enzymu wọnyi ni awọn ayẹwo ile-iwosan.