4-Nitrophenyl-2-acetamido-2-deoxy-β-D-glucopyranoside CAS: 3459-18-5

Sobusitireti Enzyme: pNAG jẹ lilo pupọ bi sobusitireti kan pato fun ọpọlọpọ awọn enzymu ti o ni ipa ninu hydrolysis ti awọn iwe adehun β-D-glucoside.Nigbati awọn enzymu wọnyi ba fọ moleku pNAG, o tu p-nitrophenol silẹ.Eyi ngbanilaaye awọn oniwadi lati ṣe iwọn ati ṣe iwọn iṣẹ ṣiṣe enzymatic naa.

Awọn atunwo Iṣẹ ṣiṣe Enzyme: hydrolysis ti pNAG nipasẹ awọn ensaemusi kan pato le ṣee wa-ri ati wiwọn spectrophotometrically.Eyi jẹ ki pNAG yẹ fun awọn idanwo iṣẹ ṣiṣe enzymu, nibiti iye p-nitrophenol ti ipilẹṣẹ jẹ ibamu taara si iṣẹ ṣiṣe enzymatic.

Ṣiṣayẹwo Ilọju-giga: pNAG ni a lo nigbagbogbo ni awọn idanwo ibojuwo-giga lati ṣe idanimọ ati ṣe afihan awọn inhibitors enzymu tabi awọn oluṣiṣẹ.Nipa iṣiro ipa ti awọn orisirisi agbo ogun lori iṣẹ enzymatic, awọn oniwadi le ṣe idanimọ awọn oludije oogun ti o ni agbara tabi awọn modulators ti iṣẹ enzymu.

Awọn ẹkọ Ikosile Gene: pNAG tun lo ninu iwadii isedale molikula lati ṣe iwadi ikosile pupọ ati ilana.Nipa wiwọn iṣẹ ṣiṣe enzymatic ti awọn enzymu kan pato nipa lilo pNAG bi sobusitireti, awọn oniwadi le ṣe iwadii ipa ti ikosile pupọ lori iṣẹ enzymu ati iṣẹ ṣiṣe.